Intracellular Protein Breakdown in a Thermophile
نویسندگان
چکیده
منابع مشابه
Intracellular Protein Breakdown in the L1210 Ascites Leukemia1
The endogenous catabolism of the cell protein of the L1210 mouse ascites tumor has been estimated from its in vitro break down to free amino acid after pulse labeling in vivowith leucine1-14C.Two general protein classes are distinguishable according to their breakdown: an unstable population representing onefifteenth of the cell protein and with an average half-life of several hours, and the st...
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The endogenous catabolism of the cell protein of the L1210 mouse ascites tumor has been estimated from its in vitro break down to free amino acid after pulse labeling in vivowith leucine1-14C.Two general protein classes are distinguishable according to their breakdown: an unstable population representing onefifteenth of the cell protein and with an average half-life of several hours, and the st...
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1. When Escherichia coli was grown exponentially in a defined medium at 35 degrees , the rate of protein breakdown was initially rapid, but decreased to 0.6%/hr. after about 30min. The latter rate was maintained for at least 3.5hr. 2. The initial rapid rate may have been due to the presence of a small protein fraction (about 1%) that was degraded with a half-life of 13min. 3. The rate of protei...
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Epitope tagging is a method of expressing proteins whereby an epitope for a specific monoclonal antibody is fused to a target protein using recombinant DNA techniques. The aim of this study was to sub-clone the peroxisomal protein (PEP) cDNA into a mammalian expression vector leading to the formation of a chimeric PEP-cDNA containing the FLAG epitope. The FLAG-PEP recombinant cDNA was construc...
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Objective(s): Mutant forms FMS-like tyrosine kinase-3 (FLT3), are reported in 25% of childhood acute lymphoid leukemia (ALL) and 30% of acute myeloid leukemia (AML) patients. In this study, drug response, growth promoting, and protein trafficking of FLT3 wild-type was compared with two active mutants (Internal Tandem Duplication (ITD)) and D835Y. Materials and Methods:FLT3 was expressed on fact...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1969
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.99.2.418-421.1969